Efficient genome editing is central to addressing biologically-relevant questions related to gene function, and to develop bacteria as delivery vehicles.
Our laboratory has made significant investments to develop genome-editing technologies for use in lactic acid bacteria. These technologies were initially developed for use in Lactobacillus reuteri, and are subsequently adapted for use in other strains and species.
For example, we have developed CRISPR-Cas-assisted recombineering in Lactobacillus reuteri, which offers exciting opportunities to edit the genome in a subtle and high-throughput manner. For more in-depth information on CRISRP-Cas engineering and single-stranded DNA recombineering in lactobacilli, please take a look at these select publications.
Our current efforts in genome-editing tools focus on the development of a liquid-based system to perform multiplex gene insertions and/or deletions.